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Characterization of protein intermolecular interactions

By 2023-01-05 No Comments

SAXS was used to study the effect of pH and protein concentration on the self-interaction process of a BSA system in solution

Bovine serum albumin (BSA) is a non-glycosylated globular protein found in bovines. It is widely used in drug delivery, immunodiagnostic procedures  and in clinical chemistry due to its low cost and readily availability. [1,2] Understanding the behavior of BSA in solution under varying conditions is crucial for assessing the stability of such systems. To characterize the protein self-interactions, SAXS data were collected for BSA in various pH solutions over  a wide range of concentrations on a laboratory instrument. SAXS enables detailed analysis of intermolecular attraction and repulsion in dilute and in concentrated solutions in near-physiological environments. SAXS can also monitor the colloidal stability of therapeutic proteins as a function of pH, ionic strength, protein concentration and excipients, protein-ligand interaction, monomer-dimer equilibrium, oligomerization and aggregation.

References:
1 Rombouts, I., Lagrain, B., Scherf, K. A., Lambrecht, M. A., Koehler, P., & Delcour, J. A. (2015). Formation and reshuffling of disulfide bonds in bovine serum albumin demonstrated using tandem mass spectrometry with collision-induced and electron-transfer dissociation. Scientific reports, 5(1), 1-12.
2 Karimi, M., Bahrami, S., Ravari, S.B., Zangabad, P.S., Mirshekari, H., Bozorgomid, M., Shahreza, S., Sori, M. and Hamblin, M.R. (2016). Albumin nanostructures as advanced drug delivery systems. Expert opinion on drug delivery, 13(11), 1609-1623.
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